11256   Bex2     Status: Cancelled

GeneBex2
ChromosomeX
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1338017
NCBI Gene ID12069
BAC ID446C3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF136,067,111136,067,132AGGAAGAAAAGCCACAGGATGC
TUP136,067,082136,067,107CAAAAGGGATCCGATTGTGGCCCTGC
TUR136,067,046136,067,067CTCCTCTAGGCACGTAGTAGTC
TDF136,066,909136,066,931CTCCATGAGCTGTCTCACATCAT
TDP136,066,932136,066,956CCCCAAACCTCTGTACGTTCTCCTC
TDR136,066,958136,066,976AGCCCCAGGGAAGGATGAG

PCR Primers

PrimerSequence
SUCAGGGAAAATGGTGGGTTG
SDTACTGAGCATCTTCCCATGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR87 bp++-
TDFTDR68 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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