11262 Slc16a14 Status: Cancelled
| Gene | Slc16a14 |
| Chromosome | 1 |
| Deletion Start | 84,929,548 |
| Deletion End | 84,907,271 |
| Deletion Size | 22,278 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,929,416 | 84,929,438 | GGTCCTCTCGTCCTTCTTTGTTC |
| TUP | 84,929,388 | 84,929,414 | CATCCTCATCATGGGCTCACAGATGGC |
| TUR | 84,929,339 | 84,929,356 | GCGGCTCTGGTGAAACTC |
| TDF | 84,907,787 | 84,907,807 | GTCCCAGGATTGGAGAGTCAG |
| TDP | 84,907,761 | 84,907,786 | AGGCCAGCCTGGTTTATGTAGTGCGT |
| TDR | 84,907,724 | 84,907,746 | CTGTTCTTTAGACAGGGCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCATCTCCTTCTCCCTCC |
| SD | AAACACTGTCACCTCTAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
