11266 Eif5a2 Status: ES cell colonies screened / QC no positives
| Gene | Eif5a2 |
| Chromosome | 3 |
| Deletion Start | 28,782,055 |
| Deletion End | 28,794,500 |
| Deletion Size | 12,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,782,606 | 28,782,630 | AAGATGACCTTGGAAGAGGTGAAAG |
| TUP | 28,782,634 | 28,782,660 | CCATTACAGTCAGAGCTGGGATTGTGC |
| TUR | 28,782,665 | 28,782,687 | GTCAGCTATGTCACTCCCTACAC |
| TDF | 28,793,725 | 28,793,745 | AAACTGGCGAAGTCCGTGAAG |
| TDP | 28,793,750 | 28,793,774 | TAAGCTGCCAGAAGGTGAACTGGGC |
| TDR | 28,793,817 | 28,793,839 | ACACAGCCTACTATGAGCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | GGTCGTGGGCACTTCTTG |
| SD | CTGACCACCAAGAGAATGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 457 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | 530 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
