11267 Defb7 Status: Cancelled
| Gene | Defb7 |
| Chromosome | 8 |
| Deletion Start | 19,523,729 |
| Deletion End | 19,497,721 |
| Deletion Size | 26,009 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,495,879 | 19,495,900 | GCTGATCTGCAGCACTTGACTT |
| TUP | 19,495,901 | 19,495,926 | CCTTTTCCTCACTCCAACCGCTAAGC |
| TUR | 19,495,972 | 19,495,998 | CAGGTAAGAATCAAACATTCTTCATGC |
| TDF | 19,497,114 | 19,497,132 | GGCCAACCTCTAGCCTCTG |
| TDP | 19,497,135 | 19,497,161 | TGTGTTTGCGCACATGCACATTCATGT |
| TDR | 19,497,259 | 19,497,281 | CAGGAGCTCAGGAAAGATTCTGG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGACTCTGAGTGTGCTC |
| SD | CAGAGACAGGAACAGATAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 6913 bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 168 bp | + | + | - |
| NeoFwd | SD | 280 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
