11271 Pgls Status: ES cell colonies screened / QC no positives
| Gene | Pgls |
| Chromosome | 8 |
| Deletion Start | 71,592,203 |
| Deletion End | 71,596,224 |
| Deletion Size | 4,022 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,592,991 | 71,593,014 | GAAACTGAGGCATGGAGGATTGAG |
| TUP | 71,593,016 | 71,593,040 | TCTGAGCACACTTGGATGACTGCCA |
| TUR | 71,593,066 | 71,593,085 | CTGCTTCAGACCCATGACAC |
| TDF | 71,595,896 | 71,595,915 | CCAATTCCACACCTCGAAGC |
| TDP | 71,595,916 | 71,595,936 | TTTGGATCCCACGGTGTGCGA |
| TDR | 71,595,989 | 71,596,008 | GGGCTCACACTGGTCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGGCCAGCAAGGCGTGTCT |
| SD | CTAGAACACAAGCAGTCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
