11272 Kcns3 Status: ES cell colonies screened / QC no positives
| Gene | Kcns3 |
| Chromosome | 12 |
| Deletion Start | 11,092,693 |
| Deletion End | 11,091,194 |
| Deletion Size | 1,500 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,092,223 | 11,092,245 | TGTTTGAGAAAGAGCTGGAGAAG |
| TUP | 11,092,184 | 11,092,212 | TGAGATTTGGTCAGCTCCGAAAGAAGATC |
| TUR | 11,092,143 | 11,092,161 | TTGGCCGACAGGCAGTAAG |
| TDF | 11,091,700 | 11,091,722 | GCTACTGCTTCTCTTCCTTTCTG |
| TDP | 11,091,676 | 11,091,699 | TGGGCATCTCCATCTTCTCTGTGC |
| TDR | 11,091,637 | 11,091,661 | GACTGGACTTGTGTTCATCTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CCATACACACAGAGACAACC |
| SD | GAATGATGCGTAAGCCGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 343 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
