11273 Cerk Status: Cancelled
| Gene | Cerk |
| Chromosome | 15 |
| Deletion Start | 86,185,645 |
| Deletion End | 86,156,594 |
| Deletion Size | 29,052 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,184,962 | 86,184,982 | GCCTTTGACTTTCCGATGGAG |
| TUP | 86,184,937 | 86,184,956 | ACCCTGCTCAGCCGCCCTCA |
| TUR | 86,184,904 | 86,184,924 | CCATGCTGGCCTAAGCTACTC |
| TDF | 86,156,910 | 86,156,933 | CCCTTTATACTACCTGGCTGTTGC |
| TDP | 86,156,886 | 86,156,909 | CAGGTAACTACTGGGCGGAGCCTA |
| TDR | 86,156,824 | 86,156,843 | AGGCGGCCTCTGTGAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GACAGACTAGTGTCTTTCAG |
| SD | TGAGAGCCAGGTCAAACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 485 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 368 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
