11274 Dupd1 Status: ES cell colonies screened / QC no positives
| Gene | Dupd1 |
| Chromosome | 14 |
| Deletion Start | 21,703,073 |
| Deletion End | 21,676,907 |
| Deletion Size | 26,167 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,702,242 | 21,702,261 | GGCCACCATTTAGGGAACAC |
| TUP | 21,702,210 | 21,702,237 | TGGTAGGCACAATGTTAAGCAACCTGCA |
| TUR | 21,702,131 | 21,702,154 | AGCTTTGTGACTCAGTTTCCTTAC |
| TDF | 21,677,093 | 21,677,111 | GGTCCTGGCCTACCTGATG |
| TDP | 21,677,064 | 21,677,090 | CCACAAGAACATGACTTTGGTGGACGC |
| TDR | 21,677,039 | 21,677,059 | GCGGTTCTTAGCCACTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AAGGAGTGTGGGTGGACTG |
| SD | GACAGGAGATTGTGGAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 424 bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
