11279 Prss12 Status: Germline transmission achieved
| Gene | Prss12 |
| Chromosome | 3 |
| Deletion Start | 123,447,162 |
| Deletion End | 123,478,151 |
| Deletion Size | 7,551 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,855,922 | 137,855,942 | TGTGCAGGAATTGCTCTGGAG |
| TUP | 137,855,895 | 137,855,920 | TGCATTGTGGGTTCAAATGGCTGAGA |
| TUR | 137,855,818 | 137,855,840 | GGAAGTGAAGGAAGCGGTTACTC |
| TDF | 137,849,421 | 137,849,438 | GAGGCCGTCTGTCAGTTC |
| TDP | 137,849,395 | 137,849,419 | CCCTGCTGTCTGGCATCCCTACCTT |
| TDR | 137,849,331 | 137,849,351 | GACTCATCTCCCTGCACCAAG |
PCR Primers
| Primer | Sequence |
| SU | AACCCAAATCACAGGCATAAG |
| SD | CCATACAGGGCACATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 14409309 bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 14370246 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
