| Gene | Prm3 |
| Chromosome | 16 |
| Deletion Start | 10,790,886 |
| Deletion End | 10,790,548 |
| Deletion Size | 339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,790,858 | 10,790,875 | TGCCAAGCTCAGCACTGG |
| TUP | 10,790,835 | 10,790,857 | CCATGGCCCAGCCCAGAACACTG |
| TUR | 10,790,799 | 10,790,820 | GCTTCTTCATGGAGGACTCGTG |
| TDF | 10,790,671 | 10,790,691 | CAAATCCCGGTGAAGGGCAAG |
| TDP | 10,790,647 | 10,790,670 | CTGCTGCTGCTGGAGCCCGAAAAG |
| TDR | 10,790,579 | 10,790,598 | GTGGCTCAGGAGTGTGTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGGACTGAGACCATCTTCCC |
| SD | TTCACTTCCCAGATCCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11281A-H6 | VGB6 | C57BL/6NTac | |
| 11281A-D6 | VGB6 | C57BL/6NTac | |
| 11281A-E2 | VGB6 | C57BL/6NTac | |
| 11281A-C8 | VGB6 | C57BL/6NTac | |
