| Gene | Fbxl22 |
| Chromosome | 9 |
| Deletion Start | 66,514,550 |
| Deletion End | 66,511,617 |
| Deletion Size | 2,934 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,514,293 | 66,514,313 | AGCATCGAGGACTGGCTCAAG |
| TUP | 66,514,271 | 66,514,292 | AGCGCCCTCCAGAGGAGCATCT |
| TUR | 66,514,213 | 66,514,232 | GCCTACCTGTTGCACACCTG |
| TDF | 66,512,490 | 66,512,510 | GGGCTCTAGGAAAGTGGGTTG |
| TDP | 66,512,468 | 66,512,489 | TGCCAGTAGTGGGCTCTGCTCA |
| TDR | 66,512,440 | 66,512,460 | CATGCACCTGTGAGGACTGAG |
PCR Primers
| Primer | Sequence |
| SU | AACACTTATCAGCTCAGAGG |
| SD | ACTATCAGTCATGCAGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 408 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11284A-C12 | VGB6 | C57BL/6NTac | |
| 11284B-C12 | VGB6 | C57BL/6NTac | |
| 11284B-B9 | VGB6 | C57BL/6NTac | |
| 11284B-F9 | VGB6 | C57BL/6NTac | |
| 11284B-D9 | VGB6 | C57BL/6NTac | |
