| Gene | Pard6a |
| Chromosome | 8 |
| Deletion Start | 105,701,727 |
| Deletion End | 105,703,417 |
| Deletion Size | 1,691 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,702,106 | 105,702,126 | TCAGTGCTGTGGTGAGAAAGG |
| TUP | 105,702,130 | 105,702,154 | TTGGGATGTCCTGACACTCCGTTCC |
| TUR | 105,702,173 | 105,702,190 | CGGAACTCGGCGTCAAAC |
| TDF | 105,703,332 | 105,703,353 | GCGAGGTGATGTTAGCGGATTC |
| TDP | 105,703,354 | 105,703,379 | AGCCTCTGACAGGCAAAGATGCAGCC |
| TDR | 105,703,385 | 105,703,406 | CATGTTCCCGCAGCCTAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGAAAGCCCTCTCTGCCTCC |
| SD | AGGAGCTGTCTTAGAAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 330 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 301 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11288A-A10 | VGB6 | C57BL/6NTac | |
| 11288A-C2 | VGB6 | C57BL/6NTac | |
| 11288A-E6 | VGB6 | C57BL/6NTac | |
| 11288A-E7 | VGB6 | C57BL/6NTac | |
| 11288A-H1 | VGB6 | C57BL/6NTac | |
| 11288A-A1 | VGB6 | C57BL/6NTac | |
