11293 Spert Status: ES cell colonies screened / QC one positive
| Gene | Spert |
| Chromosome | 14 |
| Deletion Start | 75,591,959 |
| Deletion End | 75,583,047 |
| Deletion Size | 8,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,591,187 | 75,591,206 | TCCATCTACCCTTGGCACAG |
| TUP | 75,591,150 | 75,591,177 | CCCAGCTCTCAGAATCAGCTTAGCAGTG |
| TUR | 75,591,122 | 75,591,146 | CAAGTCTCCTCAAATCACCTTTCTC |
| TDF | 75,583,542 | 75,583,560 | GGCAAGTCCACCTCCTCAC |
| TDP | 75,583,515 | 75,583,540 | CAAGGAGGAGTCCCATAACTCAGGGC |
| TDR | 75,583,487 | 75,583,509 | GAGTGGTTTGTGCTCTGATCTGG |
PCR Primers
| Primer | Sequence |
| SU | CCCATTTACAGCTGCCTAAG |
| SD | GAGGCAAGGCAAAGGAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 382 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
