11296 Speer4d Status: Cancelled
| Gene | Speer4d |
| Chromosome | 5 |
| Deletion Start | 15,619,268 |
| Deletion End | 15,623,522 |
| Deletion Size | 4,255 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTCTGGTTATCATATCTATGTTTAGGGAGAG |
| TUP | | | TATGTCTGCTTACATCTCCTGAT |
| TUR | | | CCCAGCATGAAATTTCTTCTCAGC |
| TDF | | | GGAGACAGGCAGAGGTCTTATGG |
| TDP | | | CCCATGTGTCTGCACCTTGGAGGG |
| TDR | | | GGACTGACAAAGATCAACTCAGGA |
PCR Primers
| Primer | Sequence |
| SU | TATCAGGGAACCAAAGGCTG |
| SD | AGGAAGATGGTCTCAAGAGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
