| Gene | Fbxl12 |
| Chromosome | 9 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,641,501 | 20,641,519 | CTCTGTAGCCCTGGCTGAC |
| TUP | 20,641,470 | 20,641,493 | TCTGCTTCTCCAGTGCTGGGACTA |
| TUR | 20,641,415 | 20,641,439 | CCAAGCACGAACTCACTTAAATACC |
| TDF | 20,638,541 | 20,638,560 | AGACATGCCCACACCCACTC |
| TDP | 20,638,514 | 20,638,537 | TTGTGTCCTCCTGCCTCACCATGC |
| TDR | 20,638,486 | 20,638,504 | CCTTGCACCTCAAGCACTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCCAGTCAGGCCTAGTC |
| SD | CATGAGGTAGCTATGATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
