11298 Nsg1 Status: ES cell colonies screened / QC positives
| Gene | Nsg1 |
| Chromosome | 5 |
| Deletion Start | 38,550,241 |
| Deletion End | 38,529,885 |
| Deletion Size | 20,357 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ATTCCCGCGCTGCTGAGTG |
| TUP | | | CCCTAAATCTTCGGCTGCAACTGAAACA |
| TUR | | | ACGCGCACAAGGCGTTTC |
| TDF | | | GTCCAAATGGAAGGCAGAACAG |
| TDP | | | AGTCAGTCATCTGGAGTTCTGGGTCCA |
| TDR | | | AAGCCACAGGGCAGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCATCTCTCAAGCAGAGGG |
| SD | GGTCTGCAGATAGCAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 468 bp | - | + | + |
| NeoFwd | SD | 450 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
