11299 Mlf1 Status: ES cell colonies screened / QC no positives
| Gene | Mlf1 |
| Chromosome | 3 |
| Deletion Start | 67,374,219 |
| Deletion End | 67,399,844 |
| Deletion Size | 25,626 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,374,990 | 67,375,011 | GTAAACCTTACTGGCCTGGAAC |
| TUP | 67,375,013 | 67,375,037 | CGGTGTAGATCAGGCTGACCTCACA |
| TUR | 67,375,038 | 67,375,056 | GCTGCAACAGGTGGACTTC |
| TDF | 67,399,361 | 67,399,385 | GGAATTACTACATGACAAAGTGTGC |
| TDP | 67,399,400 | 67,399,418 | ACTATTAGATACGCGTGAC |
| TDR | 67,399,429 | 67,399,446 | GCCACTGAAGGGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TACAGAGGTTGCCATAGCAG |
| SD | ACCATCACCATGGTAAGTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 442 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
