| Gene | Slc45a3 |
| Chromosome | 1 |
| Deletion Start | 131,976,944 |
| Deletion End | 131,981,808 |
| Deletion Size | 4,884 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,977,831 | 131,977,851 | ATGCCTCTTTGGCCTCCTCAC |
| TUP | 131,977,858 | 131,977,881 | TTTCCTCATCTGCATGGCAGCCAC |
| TUR | 131,977,889 | 131,977,907 | GTACTGCCTCCTCCGTCAC |
| TDF | 131,981,587 | 131,981,606 | CCTGTTCATGGGCTCCATTG |
| TDP | 131,981,608 | 131,981,631 | CCAGCTGAGCCACTCTGTCACTGC |
| TDR | 131,981,692 | 131,981,713 | GGCCAAGTCGTTCTTGTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GAATGGATTGGCTCAGCTTG |
| SD | GGTTCCTCCTTCCAACTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 598 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 127 bp | + | + | - |
| NeoFwd | SD | 450 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11303A-F1 | VGB6 | C57BL/6NTac | |
| 11303A-A12 | VGB6 | C57BL/6NTac | |
| 11303A-H12 | VGB6 | C57BL/6NTac | |
