11309 Nox4 Status: ES cell colonies screened / QC no positives
| Gene | Nox4 |
| Chromosome | 7 |
| Deletion Start | 87,247,577 |
| Deletion End | 87,246,849 |
| Deletion Size | 729 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,246,892 | 87,246,913 | ACCTCTGCCTGGTAGGATGGAG |
| TUP | 87,246,916 | 87,246,942 | CCAAGCTGGCCTGACAGACAGTCTATC |
| TUR | 87,246,968 | 87,246,989 | TCCCACCAGTACACATTCTGAG |
| TDF | 87,247,302 | 87,247,321 | CAGTGCAGGGTCTGGGTAAG |
| TDP | 87,247,323 | 87,247,347 | CCAAGTGAGGTGTCCTCAGAGCAGC |
| TDR | 87,247,383 | 87,247,405 | CACCTGGGTACATGCAATTTCAG |
PCR Primers
| Primer | Sequence |
| SU | ATGTCTGCAGCTGGACAGG |
| SD | CTTCCCTCAGACTCTGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
