| Gene | Tnfsf4 |
| Chromosome | 1 |
| Deletion Start | 161,395,585 |
| Deletion End | 161,417,360 |
| Deletion Size | 1,304 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,813,664 | 52,813,686 | CCTCTACACAGATGACGACCAAG |
| TUP | 52,813,638 | 52,813,663 | ACACTGAAGCCCACCTGGAGATCAGG |
| TUR | 52,813,614 | 52,813,631 | TGCGCCTACCACTGTTCC |
| TDF | 52,813,134 | 52,813,158 | GTACCCAGGACCAATAAATGACAAG |
| TDP | 52,813,104 | 52,813,125 | TGGTGGGCAGAATGCCAACCTA |
| TDR | 52,813,062 | 52,813,080 | AGCAGGTTCAGGCCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | CAAATATCACGCGTCAGGAG |
| SD | GTACCAGGAATGCTTTATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 108581641 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 108605146 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11315A-A9 | VGB6 | C57BL/6NTac | |
| 11315D-C2 | VGB6 | C57BL/6NTac | |
