11315 Tnfsf4 Status: ES cell colonies screened / QC positives
| Gene | Tnfsf4 |
| Chromosome | 1 |
| Deletion Start | 52,870,673 |
| Deletion End | 52,869,370 |
| Deletion Size | 1,304 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,870,508 | 52,870,530 | CCTCTACACAGATGACGACCAAG |
| TUP | 52,870,482 | 52,870,507 | ACACTGAAGCCCACCTGGAGATCAGG |
| TUR | 52,870,458 | 52,870,475 | TGCGCCTACCACTGTTCC |
| TDF | 52,869,978 | 52,870,002 | GTACCCAGGACCAATAAATGACAAG |
| TDP | 52,869,948 | 52,869,969 | TGGTGGGCAGAATGCCAACCTA
|
| TDR | 52,869,906 | 52,869,924 | AGCAGGTTCAGGCCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | CAAATATCACGCGTCAGGAG |
| SD | GTACCAGGAATGCTTTATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 379 bp | - | + | + |
| TUF | TUR | 51 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 312 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
