11319 Lrrc3b Status: ES cell colonies screened / QC no positives
| Gene | Lrrc3b |
| Chromosome | 14 |
| Deletion Start | 15,358,601 |
| Deletion End | 15,357,713 |
| Deletion Size | 889 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,358,497 | 15,358,517 | TCTGCCAGTATGTGTCCCAAG |
| TUP | 15,358,470 | 15,358,493 | TGCCTTTGCTCTTCCTCTGGAGGG |
| TUR | 15,358,439 | 15,358,461 | TGAGATTTGCATTGCTACAGGTG |
| TDF | 15,357,961 | 15,357,983 | GTCACTATGTTTGGCTGGTTCAC |
| TDP | 15,357,923 | 15,357,951 | CTCTTATGTGGTGTACTACGTGAGGCAGA |
| TDR | 15,357,876 | 15,357,899 | TGGCAGGGATTTCAAGTATTCAAG |
PCR Primers
| Primer | Sequence |
| SU | CTACTTTAGGTGGACTCCTG |
| SD | GTCTAACTAATGTTCAGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 222 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
