11321 Trim52 Status: Cancelled
| Gene | Trim52 |
| Chromosome | 14 |
| Deletion Start | 106,106,892 |
| Deletion End | 106,107,611 |
| Deletion Size | 720 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,107,135 | 106,107,153 | GAGCAGGAGCAGCACAGAG |
| TUP | 106,107,154 | 106,107,179 | CACATGCAGGACAGTGGGTCGGTCAT |
| TUR | 106,107,211 | 106,107,230 | TTCCTCGTCATCCCACTCAG |
| TDF | 106,107,462 | 106,107,483 | CATGCCCAGGTTCCTTCTCTAC |
| TDP | 106,107,485 | 106,107,506 | TCGGCCCACACCACAGGTCTTC |
| TDR | 106,107,576 | 106,107,600 | GGCAAATCTGGCGAATTATATGGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGAGGCCTACTGCGTTTG |
| SD | CTGGGATAGCACTGAGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 389 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
