11328 Prss34 Status: ES cell colonies screened / QC no positives
| Gene | Prss34 |
| Chromosome | 17 |
| Deletion Start | 25,298,497 |
| Deletion End | 25,300,055 |
| Deletion Size | 1,559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,298,604 | 25,298,624 | TCAGAGTCCTGCTCCAGATAC |
| TUP | 25,298,627 | 25,298,652 | TGGAGGTTATGCCAGTCTGTCGGTTT |
| TUR | 25,298,664 | 25,298,683 | CCAAGGCTCAGGCATCAGTG |
| TDF | 25,299,949 | 25,299,973 | GCCACTCATATTGATCCATTCACTC |
| TDP | 25,299,975 | 25,300,001 | TCCTTCTGGTCCAGCCTCACTTTCTTA |
| TDR | 25,300,022 | 25,300,043 | CAGGAGCACAAGGTAGTTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TGACTGGTCAATTCATATCTG |
| SD | TGAATAGGAAGGCAAGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
