11338 Ankrd58 Status: ES cell colonies screened / QC positives
| Gene | Ankrd58 |
| Chromosome | X |
| Deletion Start | 34,588,904 |
| Deletion End | 34,589,902 |
| Deletion Size | 999 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,589,015 | 34,589,036 | CATTGCCTTGGCAGGTACAAAG |
| TUP | 34,589,037 | 34,589,059 | TACAAGCCGTCAGGGACAGTGCC |
| TUR | 34,589,076 | 34,589,095 | CAGGGCGCTGTGGAAGATTC |
| TDF | 34,589,573 | 34,589,594 | CGTCGAGATCACAGTGGCAATC |
| TDP | 34,589,597 | 34,589,619 | CCTTGTCACTATCTGCGGCCCGA |
| TDR | 34,589,654 | 34,589,674 | GCGTTCAATCTCCCACTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGGTCCCCAGAATGTAGAC |
| SD | CTTCTCTTCCAAGGAGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 388 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11338A-B1 | VGB6 | C57BL/6NTac | |
| 11338B-B2 | VGB6 | C57BL/6NTac | |
| 11338B-C8 | VGB6 | C57BL/6NTac | |
| 11338B-F4 | VGB6 | C57BL/6NTac | |
