11342   Lrrn1     Status: ES cell colonies screened / QC no positives

GeneLrrn1
Chromosome6
Deletion Start107,567,228
Deletion End107,569,393
Deletion Size2,166
Genome Build38
MGI IDMGI:106038
NCBI Gene ID16979
BAC ID422F3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,568,116107,568,139CTGGGAATCAACAACATGGGAGAG
TUP107,568,140107,568,163CTGGTTTCCGTGGACCGCTATGCC
TUR107,568,186107,568,209GGTTATTGGTTGCTTCGAGCTTTG
TDF107,569,099107,569,117CGCCCTGGACATCTCTGAC
TDP107,569,120107,569,141TGAAACCAGCACCGCCCTTGCT
TDR107,569,159107,569,179GCTAGACTGATGACGGCAAAC

PCR Primers

PrimerSequence
SUTTCTGCAAGCATTCTGAACAC
SDAACCAAACACAAACTGGAAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev483 bp-++
TUFTUR94 bp++-
TDFTDR81 bp++-
NeoFwdSD299 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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