11346 Zfp639 Status: Cancelled
| Gene | Zfp639 |
| Chromosome | 3 |
| Deletion Start | 32,515,298 |
| Deletion End | 32,520,814 |
| Deletion Size | 5,543 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,516,001 | 32,516,021 | GCTTGCCTAGCATACCTGAGC |
| TUP | 32,516,022 | 32,516,051 | CCTGGGTTCACATCTAATCCCATCACTTGA |
| TUR | 32,516,052 | 32,516,072 | GATCTTGCCTGTCTCCACTTC |
| TDF | 32,520,044 | 32,520,065 | GAGAACCTCAGCCAGCACATTG |
| TDP | 32,520,067 | 32,520,089 | AGACACGCACTTCAGCGACCACC |
| TDR | 32,520,096 | 32,520,115 | CACGTCACACTGCTCACACC |
PCR Primers
| Primer | Sequence |
| SU | CATGGTCCACACAGACTCC |
| SD | ACGTACCCTACTCCTATAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 471 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 270 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
