11348 Tpbg Status: ES cell colonies screened / QC no positives
| Gene | Tpbg |
| Chromosome | 9 |
| Deletion Start | 85,843,983 |
| Deletion End | 85,845,327 |
| Deletion Size | 1,362 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,844,564 | 85,844,582 | CGAGGGTATGGTGGCCTTC |
| TUP | 85,844,589 | 85,844,611 | ATGGTGGCAGCAGCTCTGCGCTC |
| TUR | 85,844,629 | 85,844,648 | TAGCTCCAGGCGTGTAAGAC |
| TDF | 85,844,842 | 85,844,861 | ACGGCCTGGCTCATGTCAAG |
| TDP | 85,844,863 | 85,844,887 | TGTTCCTGGACAACAATCCCTGGGT |
| TDR | 85,844,930 | 85,844,947 | TCTGGCACCACCTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | GGGAGGAGGAGCGCCAAG |
| SD | AAGAGCTTGCTACGGTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 389 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 457 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
