11351 Rab39b Status: ES cell colonies screened / QC no positives
| Gene | Rab39b |
| Chromosome | X |
| Deletion Start | 75,578,004 |
| Deletion End | 75,574,542 |
| Deletion Size | 3,463 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,577,635 | 75,577,655 | GCCCACTTTGAGCTACCTTTG |
| TUP | 75,577,609 | 75,577,630 | CGATGCAGGAAAGAACCGCCCA |
| TUR | 75,577,553 | 75,577,575 | GCAGATTGATGGAGTTGTGGTTC |
| TDF | 75,574,910 | 75,574,933 | TCCAGAATGTCCATGAGTGGTTAG |
| TDP | 75,574,879 | 75,574,906 | AGACCAAAGTACACGTTCAGCCCTACCA |
| TDR | 75,574,852 | 75,574,874 | TTGTGACCCACCAGAACAAATAC |
PCR Primers
| Primer | Sequence |
| SU | GCGTCTACGCAGAGAATTAC |
| SD | ACAGAGCAGCCATTGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
