11354 Trim58 Status: ES cell colonies screened / QC one positive
| Gene | Trim58 |
| Chromosome | 11 |
| Deletion Start | 58,640,453 |
| Deletion End | 58,651,677 |
| Deletion Size | 11,225 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,641,288 | 58,641,307 | CAGAAGGCTGCTTTGCTCAC |
| TUP | 58,641,308 | 58,641,335 | TCATCTTCCTCTGAAGTTGCTGGACACA |
| TUR | 58,641,366 | 58,641,385 | GTCCCTTGTGCTGGTTGTTC |
| TDF | 58,651,149 | 58,651,166 | GTACAGGATGCGGAGGTC |
| TDP | 58,651,170 | 58,651,194 | AGGGATGTTCCCAGCAACCCTGAGC |
| TDR | 58,651,196 | 58,651,215 | TGCAAGGCCAGGTGTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCAGAAGAGGAGCTGCTG |
| SD | AGTAGTGTTTGATGAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 317 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
