11375 Zxdb Status: Cancelled
| Gene | Zxdb |
| Chromosome | X |
| Deletion Start | 94,798,180 |
| Deletion End | 94,795,546 |
| Deletion Size | 2,635 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,797,803 | 94,797,821 | CGGCAGCGACGACTTCTTC |
| TUP | 94,797,781 | 94,797,802 | CTGTTGCTGCTTGACCCGGTGG |
| TUR | 94,797,754 | 94,797,772 | CCGTGCCCACGGTTTCTAC |
| TDF | 94,796,089 | 94,796,107 | ACACGGCCTTGGTCAACTC |
| TDP | 94,796,062 | 94,796,088 | TGGCATCTTGACTATTGATGTGGCCTC |
| TDR | 94,796,018 | 94,796,038 | TGTTATTATCCGCAGGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACACGCTGTGTGTAAGTTC |
| SD | ATTGGTAATCCACAGATGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 249 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
