11376 Foxb2 Status: Cancelled
| Gene | Foxb2 |
| Chromosome | 19 |
| Deletion Start | 16,873,637 |
| Deletion End | 16,872,316 |
| Deletion Size | 1,322 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,873,228 | 16,873,246 | CAGGAGGTCACCTGCATCC |
| TUP | 16,873,204 | 16,873,225 | ACCATCCCCACCACGCACACCA |
| TUR | 16,873,159 | 16,873,178 | TGATGGTGGTGATGGTGTGC |
| TDF | 16,872,848 | 16,872,868 | CGTCCACCACAGGCTTCAAAC |
| TDP | 16,872,819 | 16,872,846 | TCCGTTCGCCATAGAGAACATCATCGGG |
| TDR | 16,872,764 | 16,872,781 | GCATGACCGACGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAAGACCAGAAGTTGTGAG |
| SD | AACTCTTACCTGAAAATGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 266 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
