11379 Tfpi2 Status: Cancelled
| Gene | Tfpi2 |
| Chromosome | 6 |
| Deletion Start | 3,968,321 |
| Deletion End | 3,963,332 |
| Deletion Size | 4,990 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,968,089 | 3,968,109 | CCCTGCTAGGCACCAAATCTG |
| TUP | 3,968,067 | 3,968,088 | ACCGCCTCCCTCGTTTCGTTCC |
| TUR | 3,968,013 | 3,968,030 | TGCGTCCAAGGGCAAGAG |
| TDF | 3,963,645 | 3,963,663 | GCCTTGGTTTCTCGGACAG |
| TDP | 3,963,617 | 3,963,643 | AGGCTTATACCGTCTAGCCACAGGAGA |
| TDR | 3,963,568 | 3,963,590 | TCACTCAGTTGCTCCTCTTCTAC |
PCR Primers
| Primer | Sequence |
| SU | TCAGGCTCAACTTCTCAATTC |
| SD | GTCTTCTGAAATTAGTACTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 324 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
