| Gene | Hmx1 |
| Chromosome | 5 |
| Deletion Start | 35,389,120 |
| Deletion End | 35,392,373 |
| Deletion Size | 3,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,389,973 | 35,389,990 | AGCCTGCGTTGGGTCTTC |
| TUP | 35,389,991 | 35,390,011 | TCAGGCCCAAAGGCTGAGGCT |
| TUR | 35,390,022 | 35,390,041 | GGGATGGACCCTTCAGAGTG |
| TDF | 35,391,638 | 35,391,661 | GTTTGAGTGAGGAGGATTTGAAGG |
| TDP | 35,391,662 | 35,391,687 | TGGGAGGAAGCCAGTCTCAGTGACTC |
| TDR | 35,391,712 | 35,391,731 | GGGAGAAACGGGCATAGTGG |
PCR Primers
| Primer | Sequence |
| SU | AGAAACGCGAGGGAGGCTG |
| SD | CATCGCCGCGGCTTTCCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11387B-A7 | VGB6 | C57BL/6NTac | |
| 11387B-B6 | VGB6 | C57BL/6NTac | |
| 11387B-F11 | VGB6 | C57BL/6NTac | |
| 11387B-H1 | VGB6 | C57BL/6NTac | |
| 11387B-H5 | VGB6 | C57BL/6NTac | |
| 11387B-H10 | VGB6 | C57BL/6NTac | |
