11389 Rtl1 Status: ES cell clone microinjected
| Gene | Rtl1 |
| Chromosome | 12 |
| Deletion Start | 109,595,400 |
| Deletion End | 109,590,169 |
| Deletion Size | 5,232 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,594,602 | 109,594,625 | CCCAAGACATGGAGGAATCAAGTG |
| TUP | 109,594,562 | 109,594,588 | CCAGGAGTCGAGTGATGGTTCAAACCA |
| TUR | 109,594,480 | 109,594,500 | GCTTGACTCCAGGTTGGAAAC |
| TDF | 109,590,982 | 109,591,004 | GGGCCGTTGTCCTAAACTTCTTC |
| TDP | 109,590,957 | 109,590,981 | CGTGGCCTGCTCTACTGGAAGAGTC |
| TDR | 109,590,912 | 109,590,933 | GCTGCCTTGCTCTCAACATCAC |
PCR Primers
| Primer | Sequence |
| SU | AATGCAGGAGGAATTTCTCC |
| SD | TACACAGTCTTGGCTGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 360 bp | - | + | + |
| TUF | TUR | 146 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 291 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
