| Gene | Rhox10 |
| Chromosome | X |
| Deletion Start | 38,066,600 |
| Deletion End | 38,071,740 |
| Deletion Size | 5,141 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,067,209 | 38,067,230 | CCCAACCAGTGCTCGAACTTAG |
| TUP | 38,067,235 | 38,067,256 | TTGAAGTCCTGTCGCAGCCTCC |
| TUR | 38,067,261 | 38,067,281 | GCACCGAACGAATCTGACCTG |
| TDF | 38,071,513 | 38,071,533 | ACATCTGGAACCTCGAACAAC |
| TDP | 38,071,537 | 38,071,561 | TCGGCTCAGATGAATGAAGACCCCA |
| TDR | 38,071,563 | 38,071,585 | CCTCAGGAACAGAGGTACTACTC |
PCR Primers
| Primer | Sequence |
| SU | GAATTCAGGTCCAGCTCAGG |
| SD | TTACCCTTGCTATCAGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11396A-E9 | VGB6 | C57BL/6NTac | |
| 11396A-H10 | VGB6 | C57BL/6NTac | |
