11397 Ccdc96 Status: Cancelled
| Gene | Ccdc96 |
| Chromosome | 5 |
| Deletion Start | 36,484,637 |
| Deletion End | 36,486,410 |
| Deletion Size | 1,774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,485,349 | 36,485,369 | GGGCGTCCACTGAAGAGTTTG |
| TUP | 36,485,372 | 36,485,393 | TGGACAGCGGACATGCAGAAGC |
| TUR | 36,485,441 | 36,485,459 | GGTTCCGCTCCACTAGCAG |
| TDF | 36,485,602 | 36,485,620 | GGCCATGCTGGAGGAACTG |
| TDP | 36,485,626 | 36,485,655 | ACAAGAGGCAGACGACTTAGAGTGGTACCG |
| TDR | 36,485,681 | 36,485,701 | GGTCTGCTTCTCTTGGCACTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGTAGGCCGTGCGGGC |
| SD | CAGTTTAATCACCGCACTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 332 bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 291 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
