11400 Gpr165 Status: ES cell colonies screened / QC positives
| Gene | Gpr165 |
| Chromosome | X |
| Deletion Start | 96,713,982 |
| Deletion End | 96,717,560 |
| Deletion Size | 3,579 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,714,383 | 96,714,401 | CACCGGCCTCTTCGTTGTC |
| TUP | 96,714,407 | 96,714,432 | TTCTGTGACCAACATGATGCTCGCTC |
| TUR | 96,714,451 | 96,714,472 | TCAGTAGCCCAGCTTACCATAG |
| TDF | 96,716,833 | 96,716,855 | CCTAGCAACACAACAGAGGAATC |
| TDP | 96,716,856 | 96,716,879 | TGCCTGCCTCCCAAGTTTCCCATA |
| TDR | 96,716,905 | 96,716,925 | CAGAAAGGTGCCCAGGTCAAG |
PCR Primers
| Primer | Sequence |
| SU | ATCTCCAGTTTGGAGCAGGT |
| SD | CATCCTCACTACCATAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 470 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
