11404 Ceacam11 Status: Cancelled
| Gene | Ceacam11 |
| Chromosome | 7 |
| Deletion Start | 18,009,953 |
| Deletion End | 17,978,589 |
| Deletion Size | 31,365 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,972,587 | 17,972,608 | GGGAGGGCCATTGAGATGATTG |
| TUP | 17,972,611 | 17,972,632 | TGGTCATGCTGCTTGCTGCCAA |
| TUR | 17,972,639 | 17,972,660 | TGCTGGGAATTGAACTGATGTC |
| TDF | 17,977,779 | 17,977,799 | GGAGGCTGGGACAGATTTCTC |
| TDP | 17,977,808 | 17,977,836 | ACTCTATCCTTGGCATTTGACTCTGCTGA |
| TDR | 17,977,876 | 17,977,898 | CAGAGGGTGCATGGGAATGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GGACTGAGGCATAATGAGAG |
| SD | GAGAAGTTGCATCATTCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 38088 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
