11405 Slc30a10 Status: ES cell colonies screened / QC no positives
| Gene | Slc30a10 |
| Chromosome | 1 |
| Deletion Start | 185,455,049 |
| Deletion End | 185,464,576 |
| Deletion Size | 9,528 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 185,455,930 | 185,455,954 | TCTTGACCCTGTAGTCCTTATCTTC |
| TUP | 185,455,956 | 185,455,983 | CCTCTCCTGTGGCAGTATTGATCTTGGA |
| TUR | 185,455,997 | 185,456,018 | GAAATGCTCCGTTGCCCAATAG |
| TDF | 185,464,378 | 185,464,396 | GCCTCTGGCTCTCGTCAAC |
| TDP | 185,464,402 | 185,464,423 | TGCAGAGCACAATGGCAGGTCC |
| TDR | 185,464,444 | 185,464,465 | GGCCACTTCTGGAGCTTCTATG |
PCR Primers
| Primer | Sequence |
| SU | CACCAGCCAAATCAAGCTTG |
| SD | GAGTCCTTCAGCACTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
