| Gene | Acvr2b |
| Chromosome | 9 |
| Deletion Start | 119,427,487 |
| Deletion End | 119,433,682 |
| Deletion Size | 6,196 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,428,285 | 119,428,303 | TCGTCCTGCTGGCCTTCTG |
| TUP | 119,428,306 | 119,428,332 | TGTATCGTCATCGGAAGCCTCCCTACG |
| TUR | 119,428,355 | 119,428,373 | CCAACGCTGGCACTGTCTC |
| TDF | 119,433,283 | 119,433,301 | GCGTGACCATCGAGGAGTG |
| TDP | 119,433,307 | 119,433,331 | ACCATGATGCAGAGGCTCGCCTTTC |
| TDR | 119,433,342 | 119,433,363 | TCAGGGATACCCGCTCTTCTAC |
PCR Primers
| Primer | Sequence |
| SU | GAAAGAGCGCAGGAAGGTC |
| SD | CCGTGTAACAGTCTTCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11407A-B9 | VGB6 | C57BL/6NTac | |
| 11407A-D5 | VGB6 | C57BL/6NTac | |
| 11407A-G6 | VGB6 | C57BL/6NTac | |
