11407   Acvr2b     Status: ES cell colonies screened / QC positives

GeneAcvr2b
Chromosome9
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:87912
NCBI Gene ID11481
BAC ID323B9
BAC LibrarybMQ
CassetteTM-ZEN-Ub1
Clones AvailableYes

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTCGTCCTGCTGGCCTTCTG
TUPTGTATCGTCATCGGAAGCCTCCCTACG
TURCCAACGCTGGCACTGTCTC
TDFGCGTGACCATCGAGGAGTG
TDPACCATGATGCAGAGGCTCGCCTTTC
TDRTCAGGGATACCCGCTCTTCTAC

PCR Primers

PrimerSequence
SUGAAAGAGCGCAGGAAGGTC
SDCCGTGTAACAGTCTTCAAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11407A-B9VGB6C57BL/6NTac
11407A-D5VGB6C57BL/6NTac
11407A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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