11411 Ankrd33 Status: Cancelled
| Gene | Ankrd33 |
| Chromosome | 15 |
| Deletion Start | 101,115,867 |
| Deletion End | 101,119,756 |
| Deletion Size | 3,890 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,116,605 | 101,116,627 | GCTTTGGTTCCATTGTGGCTCTG |
| TUP | 101,116,629 | 101,116,653 | TCAGCTGCTGTCCTTTCCTGGATGT |
| TUR | 101,116,664 | 101,116,683 | GCTGTGTTCCCGTCTTTGTC |
| TDF | 101,118,959 | 101,118,979 | CGTGGCTCACTAACCAATCTG |
| TDP | 101,118,980 | 101,119,000 | TCACACTGCGCCACTTGCAGG |
| TDR | 101,119,037 | 101,119,054 | GCCCATTCCAAGGCTGTC |
PCR Primers
| Primer | Sequence |
| SU | CAATGGCAGAGAGTACAGTC |
| SD | TTCATGTGAGAGCAGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 326 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 279 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
