11415 Mill1 Status: ES cell colonies screened / QC no positives
| Gene | Mill1 |
| Chromosome | 7 |
| Deletion Start | 18,245,613 |
| Deletion End | 18,265,063 |
| Deletion Size | 19,451 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,245,633 | 18,245,651 | AGCCCTAGCTGCGATCCAC |
| TUP | 18,245,653 | 18,245,677 | TGTGGATAGTGTACCTGCTCCTGGA |
| TUR | 18,245,710 | 18,245,730 | GCAAGGTCCCTCTTCCTGTTC |
| TDF | 18,264,771 | 18,264,791 | GGGTGTCCATTCGGGTTCTTC |
| TDP | 18,264,793 | 18,264,819 | TGGACAGGAATCACAGTTCAGCTGCAA |
| TDR | 18,264,844 | 18,264,864 | TCAGTGGCAGCAGGTTCATTG |
PCR Primers
| Primer | Sequence |
| SU | AGCATCCTGAGCACCTCTG |
| SD | CTCTCCATTCACCAGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 274 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
