11416 Mex3d Status: Cancelled
| Gene | Mex3d |
| Chromosome | 10 |
| Deletion Start | 80,387,417 |
| Deletion End | 80,381,070 |
| Deletion Size | 6,348 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,386,572 | 80,386,594 | CGTATGTATTGGTGGGAGGAAGG |
| TUP | 80,386,547 | 80,386,569 | CAAGCTGTGGCCCGTTCTAGCCC |
| TUR | 80,386,523 | 80,386,543 | GGGTCTCCTGGTCACATTCAC |
| TDF | 80,381,482 | 80,381,501 | GTCCTCCACCCTAGACACTG |
| TDP | 80,381,457 | 80,381,479 | CCCTCGGAAGGCAACCACAAGCC |
| TDR | 80,381,435 | 80,381,454 | GGAGTTCGCTGCTGTGGTAG |
PCR Primers
| Primer | Sequence |
| SU | CAAACACGTGACTGCCGGC |
| SD | CACTGTTAAATCCAGTGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 469 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
