11420 Cma2 Status: Cancelled
| Gene | Cma2 |
| Chromosome | 14 |
| Deletion Start | 55,971,468 |
| Deletion End | 55,973,839 |
| Deletion Size | 2,372 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,971,764 | 55,971,787 | CCTAATGGATTGGAAACCCAGAAG |
| TUP | 55,951,979 | 55,952,008 | TGTGTTCTGAATGAAGGATGATGGTGGACA |
| TUR | 55,971,916 | 55,971,933 | CCTGCGACTGCTCCAAGT |
| TDF | 55,973,333 | 55,973,352 | GAGGAGGAAGTGTGCCAAGC |
| TDP | 55,973,354 | 55,973,378 | TCTCTCTGTGGCAGTACCCAATGCC |
| TDR | 55,973,444 | 55,973,465 | GGAAGGTTAAGGAAGGGCTTCG |
PCR Primers
| Primer | Sequence |
| SU | GCAGTCACCACCTCATCATG |
| SD | TTAGGTCTTTACTGAGGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 170 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
