11425 Dusp23 Status: Cancelled
| Gene | Dusp23 |
| Chromosome | 1 |
| Deletion Start | 172,632,879 |
| Deletion End | 172,631,563 |
| Deletion Size | 1,317 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,632,700 | 172,632,718 | CCTCACGCTGCACCGAATG |
| TUP | 172,632,674 | 172,632,697 | CATCCCTGACTTTTGCCCGCCGTC |
| TUR | 172,632,632 | 172,632,651 | GCCTCGTCCACGATCTTCAC |
| TDF | 172,631,733 | 172,631,751 | GCTTTGGCCGCAGGAGATG |
| TDP | 172,631,711 | 172,631,732 | CCATTGCTGAGATCCGGCGCCT |
| TDR | 172,631,650 | 172,631,670 | TCGCTGGTAGAACTGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGCCTTGGAGCCGTCTTAG |
| SD | AGTGCTGTGTACATGCTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 320 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 244 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
