11429 Coro2a Status: Cancelled
| Gene | Coro2a |
| Chromosome | 4 |
| Deletion Start | 46,563,092 |
| Deletion End | 46,539,107 |
| Deletion Size | 23,986 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,562,199 | 46,562,222 | GGAGGGTGACGACTTCAAAGATAG |
| TUP | 46,562,171 | 46,562,195 | CCCTCATCACAACGCAGTGGGTGTA |
| TUR | 46,562,145 | 46,562,163 | TTAGCCGGTCTGCTGTTAC |
| TDF | 46,539,710 | 46,539,728 | GCTTAGGGCAGCGGGTTAC |
| TDP | 46,539,682 | 46,539,705 | TATCCACAAGCTGGAGGCGGACAG |
| TDR | 46,539,640 | 46,539,661 | GGATGAGATCCTGGGCTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGATAGCCTGCTCACCTGG |
| SD | CATGTGGCTGATTCTGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 423 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 473 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
