11432 Tigd4 Status: Cancelled
| Gene | Tigd4 |
| Chromosome | 3 |
| Deletion Start | 84,593,762 |
| Deletion End | 84,595,320 |
| Deletion Size | 1,559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,594,379 | 84,594,401 | GGGAAACATGCTCAGTTGGAAAG |
| TUP | 84,594,402 | 84,594,427 | TTGTGCAAAGACAGGATAACGCTGGC |
| TUR | 84,594,436 | 84,594,459 | GCTTCTCAGAGCCATCCATATTTG |
| TDF | 84,595,035 | 84,595,052 | TACGCTGCCCTGGATGAG |
| TDP | 84,595,055 | 84,595,078 | CCTGGAGACTTGTGAAGCCACCCA |
| TDR | 84,595,104 | 84,595,127 | ACCAGTTTCGTCTTGCTTACTTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGGTGGTGCCTCTTCTAAC |
| SD | CAAGTATAAAGCCTCTGAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
