11434 Diras2 Status: Cancelled
| Gene | Diras2 |
| Chromosome | 13 |
| Deletion Start | 52,508,266 |
| Deletion End | 52,507,652 |
| Deletion Size | 615 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,508,142 | 52,508,162 | CATCCCGACTGTGGAAGACAC |
| TUP | 52,508,118 | 52,508,140 | TACCGGCAGGTGATCAGCTGTGA |
| TUR | 52,508,029 | 52,508,048 | CGTGGCCTTTGGAGATGGAC |
| TDF | 52,507,836 | 52,507,854 | TAGCCCGCACATGGAAGTG |
| TDP | 52,507,810 | 52,507,832 | CTTCATGGAGACGTCCGCCAAGC |
| TDR | 52,507,725 | 52,507,744 | TTCTTGCCGTCGATCTGGAG |
PCR Primers
| Primer | Sequence |
| SU | TTTGAGCTGTGGAAGTGGC |
| SD | TGCATCGCAGGTTCTGCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | 284 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
