11436 Sox21 Status: ES cell colonies screened / QC no positives
| Gene | Sox21 |
| Chromosome | 14 |
| Deletion Start | 118,235,632 |
| Deletion End | 118,234,776 |
| Deletion Size | 857 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,235,540 | 118,235,560 | ATGGCCCAGGAGAACCCTAAG |
| TUP | 118,235,511 | 118,235,534 | CAACTCGGAGATCAGCAAGCGCCT |
| TUR | 118,235,414 | 118,235,434 | GTCGGGATGCTCCTTCATGTG |
| TDF | 118,234,916 | 118,234,935 | ACATGATCCCGTGCAACTGC |
| TDP | 118,234,899 | 118,234,915 | AGCGCGTGGCCCAGCCC |
| TDR | 118,234,857 | 118,234,877 | ATGCCTGGCAGCAGGATGTAG |
PCR Primers
| Primer | Sequence |
| SU | GTCGTCTGGTCCTCTCTTC |
| SD | TGATGCTGCGAAATCCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 274 bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 276 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
