11440 Zfp784 Status: ES cell colonies screened / QC no positives
| Gene | Zfp784 |
| Chromosome | 7 |
| Deletion Start | 5,038,409 |
| Deletion End | 5,035,630 |
| Deletion Size | 2,780 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,038,026 | 5,038,045 | TGGATGGTTGGGAAGGTGAC |
| TUP | 5,037,997 | 5,038,025 | CCCAAAGGAGAGCTATAATTCGGTCACCG |
| TUR | 5,037,936 | 5,037,955 | TCTCCTCCCTTGGGCATTTG |
| TDF | 5,035,875 | 5,035,896 | GGCCCTACCACTGTAGTATCTG |
| TDP | 5,035,852 | 5,035,874 | TGGCAAAGGCTTCACGCAGTCCT |
| TDR | 5,035,827 | 5,035,846 | ATGCGGGCATGACCACTCAG |
PCR Primers
| Primer | Sequence |
| SU | AGACATTGGAATGCGTCGC |
| SD | TCTGGGAGACCAGAACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 263 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
